Physicochemical properties of lactoferrin under oxidative/halogenative stress
https://doi.org/10.29235/1561-8323-2019-63-2-189-197
Abstract
Lactoferrin (Lf) was discovered in the thirties of the twentieth century. Since that time a number of useful properties of Lf (antibacterial, antiviral, pro- and anti-inflammatory, etc.) have been found. That’s why Lf became a promising candidate for pharmaceuticals use. The concentration of Lf strikingly increases in inflammatory focuses due to neutrophil degranulation. At the same time, activated neutrophils starts to generate reactive oxygen and halogen species (ROS and RHS), which leads to the development of oxidative/halogenative stress. In this work, using the fluorescence analysis we found the change of the Lf structure and properties in the inflammation conditions (under oxidatives/halogenative stress). We use two forms of Lf – human Lf, excreted from human milk, and recombinant Lf, excreted from milk of transgenic goats. It was established that the amino acids of Lf (decreasing the number of tryptophanils and primary amines) and protein restructuring undergo modification under the HOCl action, while H2O2 has no influence. These changes in the molecule under the HOCl treatment result in decreasing the iron-binding capacity of Lf.
About the Authors
M. S. Terekhova
Belarusian State University.
Belarus
Belarus
Terekhova Maria Sergeevna – Trainee of Junior researcher.
5, Bobruiskaya Str., 220030, Minsk.
D. V. Grigorieva
Belarusian State University.
Belarus
Belarus
Grigorieva Daria Vladimirovna – Ph. D. (Biology), Senior researcher.
5, Bobruiskaya Str., 220030, Minsk.
I. V. Gorudko
Belarusian State University.
Belarus
Belarus
Gorudko Irina Vladimirovna – Ph. D. (Biology), Assistant professor, Leading researcher.
5, Bobruiskaya Str., 220030, Minsk.
I. V. Semak
Belarusian State University.
Belarus
Belarus
Semak Igor Victorovich – Ph. D. (Biology), Assistant professor, Head of the Department.
5, Bobruiskaya Str., 220030, Minsk.
A. V. Sokolov
Institute of Experimental Medicine.
Russian Federation
Russian Federation
Sokolov Aleksey Victorovich – D. Sc. (Biology), Professor, Head of the Laboratory.
12, Academic Pavlov Str., 197376, Saint-Petersburg.
O. M. Panasenko
Federal Research and Clinical Center of Physical-Chemical Medicine.
Russian Federation
Russian Federation
Panasenko Oleg Mikhailovich – D. Sc. (Biology), Professor, Head of the Laboratory.
1a, Malaya Pirogovskaya Str., 119435, Moscow.
S. N. Cherenkevich
Belarusian State University.
Russian Federation
Russian Federation
Cherenkevich Sergey Nikolaevich – Academician, D. Sc. (Biology), Professor.
5, Bobruiskaya Str., 220030, Minsk.
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