Influence of exogenous β-1,3-glucane on the pH level of apoplast and cytoplasm in healthy and Bipolaris sorokiniana (Sacc.) Shoem. seedlings of barley (Hordeum vulgare L.)-infected tissues
https://doi.org/10.29235/1561-8323-2019-63-3-317-324
Abstract
Еffects of β-1,3-glucan from euglena (Euglena gracilis) were studied in vivo by evaluating pH changes outside and inside the cell in the tissues of the 7-day leaves of barley seedlings with the use of pH-sensitive probes of FITC-dextran and pyranine. It was found that the incubation of barley leaves separated from the roots in the solution of β-1,3-glucan (0.01 %) for 40 min did not cause acidification of cytoplasm as a typical nonspecific plant response to wound stress. The inoculation of intact seedlings with Bipolaris sorokiniana (Sacc.) Shoem. spores resulted in alkalization of apoplast by 1.7 pH units, and pretreatment of seedlings with β-1,3-glucan a day before the fungal infection promoted its acidification (1.04 pH units) compared to the infected variant, indicating an increased performance of ATPase, which pumped protons from cytoplasm into apoplast. The conducted studies contribute to the selection of optimal concentrations of β-1,3-glucan for immunomodulatory mixtures.
About the Authors
G. E. Savchenko
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Savchenko Galina Evseevna – Ph. D. (Biology), Associated professor, Senior researcher
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
T. S. Bachyshcha
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Bachyshcha Tatsiana Sergeevna – Junior researcher
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
L. F. Kabashnikova
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Kabashnikova Liudmila Fedorovna – Corresponding Member, D. Sc. (Biology), Associated professor, Head for the Laboratory
27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
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