INFLUENCE OF GOLD NANOPARTICLES ON AGGREGATION OF MITOCHONDRIAL ASPARTATE AMINITRANSFERASE
Abstract
We have studied the dependence of aggregation of mitochondrial aspartate aminotransferase (mAspAT) from the concentration of gold nanoparticles (AuNP). It has been shown that AuNPs decreased the aggregation of mAspAT in the temperature range from 55 to 73 °C. The maximal anti-aggregational effect of AuNP reached 56 % and was observed at 60 °C. Increase of AuNP concentration led to a decrease of the constant rate of enzyme aggregation. We suggest here that interaction between Au-NPs and mAspAT increases conformational stability of the enzyme molecule. It also reduces the probability of polypeptide chain unfolding, which causes exposure of hydrophobic patches on the protein surface resulting in intra molecular adhesion followed by the protein aggregation.
About the Authors
A. E. SALEM
Белорусский государственный университет, Минск
Belarus
Belarus
M. V. SHOLUKH
Белорусский государственный университет, Минск
Belarus
Belarus
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