Analysis of the intralinear polymorphism and the homozygosity rate of triticale doubled haploid lines by microsatellite markers
https://doi.org/10.29235/1561-8323-2020-64-2-199-208
Abstract
We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- (Xgwm186, Xgwm291, Xgwm595) and B- (Xgwm371, Xgwm540, Xgwm554, Xgwm234), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186, Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro.
Supporting agencies: The work was carried out in the context of the scientific event 5 “To develop the homozygosity biotechnology of genome in the triticale on the basis of androgenesis in vitro and DNA marking and to make a highproductive grade” subprogramme 1 “Innovation biotechnologies–2020” GP “High technologies and technique” in 2016–2020.
About the Authors
A. V. Lagunovskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Lahunovskaya Alena V. – Researcher.
27, Akademicheskaya Str., 220072, Minsk
A. A. Buracova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Burakova Aryna A. – Junior researcher.
27, Akademicheskaya Str., 220072, Minsk
V. N. Bushtevich
Research and Practical Center of National Academy of Sciences of Belarus for Arable Farming
Belarus
Belarus
Bushtevich Victar N. – Ph. D. (Agrarian), Assistant professor, Head of the Laboratory.
1, Timiryazev Str., 222160, Zhodino
V. I. Sakovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Sakovich Valiantsina I. – Researcher.
27, Akademicheskaya Str., 220072, Minsk
V. A. Lemesh
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Lemesh Valiantsina A. – Ph. D. (Biology), Assistant professor, Head of the Laboratory.
27, Akademicheskaya Str., 220072, Minsk
S. I. Gryb
Research and Practical Center of National Academy of Sciences of Belarus for Arable Farming
Belarus
Belarus
Gryb Stanislau I. – Academician, D. Sc. (Agrarian), Professor, Chief researcher.
1, Timiryazev Str., 222160, Zhodino
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