Characteristic spectra of class I and II HLA-alleles in patients with different clinical forms of juvenile idiopathic arthritis in the Republic of Belarus
https://doi.org/10.29235/1561-8323-2020-64-2-209-216
Abstract
The genes of the major histocompatibility complex (HLA) play a significant role in the genetic predisposition to juvenile idiopathic arthritis (JIA) and determine up to 18 % of disease risk. This work was aimed to reveal associations of the HLA characteristic pattern with two clinically different forms of JIA in the Belarusian population. 24 patients diagnosed with systemic JIA, 24 patients with oligoarticular JIA and 24 healthy controls were included into the study. High-throughput HLA typing for 11 loci was performed using TruSight HLA v2 Sequencing Panel (Illumina) on the MiSeq system. DQB1*04:02:01 (p = 0.026; OR 5.88 [1.20–28.72]), DRB1*08:01:01 (p = 0.07; OR 3.94 [1.01–15.39]) and DR8-haplotype (p = 0.006; OR 3.95 [1.34–11.63]) frequencies were significantly higher in patients with oligoarthritis but not systemic JIA when compared with controls. While DQA1*05:01:01 and DQB1*02:01:01 alleles showed a protective effect against both systemic (p = 0.007, OR 0.08 [0.009–0.65]; p = 0.01, OR 0.09 [0.01–0.83]) and oliarticular JIA (p = 0.026, OR 0.16 [0.03–0.79]; p = 0.046, OR 0.2 [0.04–1.00], the negative association of the DRB1*03:01 allele was revealed only for systemic JIA (p = 0.03, OR 0.11 [0.01–0.88]). Thus, the obtained results suggest that different HLA-patterns are involved into the formation of various JIA subtypes.
Supporting agencies: The study was performed within the framework of task 6.4 С-G Scientific and Technical Program of the Union State “DNA identification”.
About the Authors
H. A. Yatskiu
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Yatskiu Hanna A. – Researcher.
27, Akademicheskaya Str., 220072, Minsk
M. V. Zlotnikova
Republican Scientific and Practical Center of Transfusiology and Medical Biotechnology
Belarus
Belarus
Zlotnikova Maryia V. – Ph. D. (Medicine), Head of the Laboratory.
160, Dolginovskiy tract, 220053, Minsk
A. V. Sukalo
Belarusian State Medical University
Belarus
Belarus
Sukalo Alexandr V. – Academician, D. Sc. (Medicine), Professor, Head of the Department.
83, Dzerzhinski Ave., 220116, Minsk
R. I. Goncharova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Goncharova Roza I. – D. Sc. (Biology), Professor, Head of the Laboratory.
27, Akademicheskaya Str., 220072, Minsk
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