Molecular nature of cytotoxicity of zinc oxide nanostructures
https://doi.org/10.29235/1561-8323-2020-64-4-448-456
Abstract
A comparative analysis of the molecular process of nanostructured zinc oxide of different shape (rod and spherical particle) and zinc chloride as a source of free ions on human lymphocytes was carried out. Our data indicated that ZnO nanostructures induced a decrease in the lymphocyte viability and an increase in the intracellular labile Zn2+ pool in a concentration-dependent manner after 20 and 40 h incubation with cells. It was found that ZnO NRs have the lowest cytotoxicity, but ZnCl2 - the highest. The Zn-mediated activation of free radical processes - ROS accumulation in the cells -is a possible mechanism of the cytotoxic action of these agents. Thus, the shape of ZnO nanomaterial and its ability “to shed” zinc ions make the main contribution to the detected molecular and membrane process on human lymphocytes in vitro.
Keywords
human lymphocytes,
zinc oxide nanorods and nanoparticles,
viability,
a label zinc pool,
reactive oxygen species
Supporting agencies: The authors are grateful to the Head of the Laboratory of Institute of Atomic Physics and Spectroscopy of the University of Latvia (Riga, Latvia) Ph. D. R. V. Viter for the provided samples of nanostructured zinc oxide. This work was supported by the BRFFR (grant Б17-128).
About the Authors
Yu. M. Harmaza
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Harmaza Yuliya M. - Ph. D. (Biology), Leading researcher. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
A. V. Tamashevski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Tamashevski Alexander V. - Ph. D. (Biology), Leading researcher. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
E. I. Slobozhanina
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Slobozhanina Ekaterina I. - Corresponding Member, D. Sc. (Biology), Professor, Head of the Laboratory. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
References
1. Wang B., Zhang J., Chen C., Xu G., Qin X., Hong Y., Bose D. D., Qiu F., Zou Z. The size of zinc oxide nanoparticles controls its toxicity through impairing autophagic flux in A549 lung epithelial cells. Toxicology Letters, 2018, vol. 285, pp. 51-59. https://doi.org/10.1016/j.toxlet.2017.12.025
2. Chen P., Wang H., He M., Chen B., Yang B., Hu B. Size-dependent cytotoxicity study of ZnO nanoparticles in HepG2 cells. Ecotoxicology and Environmental Safety, 2019, vol. 171, pp. 337-346. https://doi.org/10.1016/j.ecoenv.2018.12.096
3. Wiesmann N., Kluenker M., Demuth P., Brenner W., Tremel W., Brieger J. Zinc overload mediated by zinc oxide nanoparticles as innovative anti-tumor agent. Journal of Trace Elements in Medicine and Biology, 2019, vol. 51, pp. 226-234. https://doi.org/10.1016/j.jtemb.2018.08.002
4. Theerthagiri J., Salla S., Senthil R. A., Nithyadharseni P., Madankumar A., Arunachalam P., Maiyalagan T., Kim H. S. A review on ZnO nanostructured materials: energy, environmental and biological applications. Nanotechnology, 2019, vol. 30, no. 39, pp. 392001. https://doi.org/10.1088/1361-6528/ab268a
5. Harmaza Y. M., Tamashevski A. V., Goncharova N. V., Slobozhanina E. I. Influence of intracellular level of zinc in human erythrocytes on the redistribution of phosphatidylserine and their viability. Novosti mediko-biologicheskih nauk = News of biomedical sciences, 2011, vol. 3, no 1, pp. 90-95 (in Russian).
6. Harmaza Y. M., Slobozhanina E. I. Zinc essentiality and toxicity. Biophysical aspects. Biophysics, 2014, vol. 59, no. 2, pp. 264-275. https://doi.org/10.1134/s0006350914020092
7. Singh S. Zinc oxide nanoparticles impacts: cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity. Toxicology Mechanisms and Methods, 2019, vol. 29, no. 4, pp. 300-311. https://doi.org/10.1080/15376516.2018.1553221
8. Harmaza Yu. M., Tamashevski A. V., Slobozhanina E. I. Membrane effects of zinc oxide nanorods and nanoparticles in human lymphocytes. Doklady Natsional'noi akademii nauk Belarusi = Doklady of the National Academy of Sciences of Belarus, 2019, vol. 63, no. 1, pp. 72-78 (in Russian). https://doi.org/10.29235/1561-8323-2019-63-1-72-78
9. Tabas I. Secretory sphingomyelinase. Chemistry and Physics of Lipids, 1999, vol. 102, no. 1-2, pp. 123-130. https://doi.org/10.1016/s0009-3084(99)00080-8
10. Gee K. R., Zhou Z. L., Ton-That D., Sensi S. L., Weiss J. H. Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes. Cell Calcium, 2002, vol. 31, no. 5, pp. 245-251. https://doi.org/10.1016/s0143-4160(02)00053-2
11. Harmaza Yu. M., Tamashevski A. V., Kanash Yu. S., Zubritskaya G. P., Kutko A. G., Slobozhanina E. I. The role of intracellular zinc in H2O2-induced oxidative stress in human erythrocytes. Biophysics, 2016, vol. 61, no. 6, pp. 950-958. https://doi.org/10.1134/s200206350916060087
12. Harmaza Yu. M., Tamashevski A. V. Zn-deficient states in human erythrocytes in vitro and free radical processes. Zhurnal Belorusskogo gosudarstvennogo universiteta. Ekologiya = Journal of Belarusian State University. Ecology, 2017, vol. 3, pp. 54-63 (in Russian).
13. Kim Y. H., Kim E. Y., Gwag B. J., Sohn S., Koh J. Y. Zinc-induced cortical neuronal death with features of apoptosis and necrosis: mediation by free radicals. Neuroscience, 1999, vol. 89, no. 1, pp. 175-182. https://doi.org/10.1016/s0306-4522(98)00313-3
14. Choi D. W., Koh J. Y. Zinc and brain injury. Annual Review of Neuroscience, 1998, vol. 21, no. 1, pp. 347-375. https://doi.org/10.1146/annurev.neuro.21.1.347
15. Trayner I. D., Rayner A. P., Freeman G. E., Farzaneh F. Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123). Journal of Immunological Methods, 1995, vol. 186, no. 2, pp. 275-284. https2://doi.org/10.1016/0022-1759(95)00152-z
Review
Views: 776
Email this article 