On proteolytic activity of subcellular fractions of Chlorella vulgaris strain C 111 IBCE C-19 cell
https://doi.org/10.29235/1561-8323-2020-64-6-694-701
Abstract
Subcellular particles of Chlorella vulgaris C 111 IBCE C-19 were isolated by differential centrifugation in sucrose density gradient. It was stated for the first time, that these particles can cleave casein, gelatin, fibrinogen and hemoglobin at pH 7.4 and 9.0. Using group-specific proteinase inhibitors, a set of serine-, cysteine- and metalloproteinases was identified in this material. However, the detection of this set of proteolytic enzymes is only possible when several different proteins are used as substrates. In some cases, virtually all of the used arsenal of group-specific proteinase inhibitors proved to be little effective. This suggests that these are proteinases of a different nature than those listed above, which requires further perspective research.
About the Authors
I. A. Ilyuchyk
Polessky State University
Belarus
Belarus
Ilyuchyk Irina A. – Postgraduate student, Senior lecturer
23, Dnieprovskaya Flotiliya Str., 225710, Pinsk
V. N. Nikandrov
Polessky State University
Belarus
Belarus
Nikandrov Vitaliy N. – D. Sc. (Biology), Professor
23, Dnieprovskaya Flotiliya Str., 225710, Pinsk
References
1. Rozhdestvenskaya L. N., Bychkova E. S., Bychkov A. L. Analysis of challenges and current trends in development technology in the protein market. Pishchevaya promyshlennost’ = Food processing Industry, 2018, no. 5, pp. 42–47 (in Russian).
2. Waghmare A. G., Salve M. K., Leblanc J. G., Arya S. S. Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa. Bioresources and Bioprocessing, 2016, vol. 3, no. 16, pp. 1–11. https://doi.org/10.1186/s40643-016-0094-8
3. Sorokina K. N., Yakovlev V. A., Piligaev V. A., Kukushkin R. G., Pel’tek S. E., Kolchanov N. A., Parmon V. N. Potential of microalgae as a source of bioenergy. Catalysis in Industry, 2012, vol. 4, no. 3, pp. 63–72 (in Russian).
4. Nikandrov V. N., Ilyuchyk I. A. Physico-chemical features of proteolytic processes realization of Chlorella vulgaris cell. Aktual’nye voprosy biologicheskoi fiziki i khimii [Topical issues of biological physics and chemistry], 2018, vol. 3, no. 3, pp. 654–664 (in Russian).
5. Ilyuchyk I. A., Nikandrov V. N. Proteolytic activity changes ofhomogenates of Chlorella vulgaris cells and culture function-metabolic reorganizations at the growth in the MnCl2 presence. Vestnik Polesskogo gosudarstvennogo universiteta. Seriya prirodovedeniya = Bulletin of Polessky State University. Series in natural sciences, 2018, no. 2, pp. 202–208. https://doi.org/10.1134/s2070050412030117
6. Ilyuchik I. A., Nikandrov V. N. Features of the organization of the proteolysis system in Chlorella vulgaris cells. Tezisy dokladov Mezhdunarodnoi nauchnoi konferentsii, trinadtsatogo s’ezda Belorusskogo obshchestvennogo ob’edineniya fotobiologov i biofizikov «Molekulyarnye, membrannye i kletochnye osnovy funktsionirovaniya biosistem» [Abstracts of the International Scientific Conference, the Thirteenth Congress of the Belarusian Public Association of Photobiologists and Biophysicists “Molecular, membrane and cellular bases of the functioning of biosystems”]. Minsk, 2018, p. 112 (in Russian).
7. Findlay J. B. C., Evans W. H. Biological membranes. A practical approach. IRL Press, 1987. 425 p.
8. Bonner A., Varner J. E., eds. Plant Biochemistry. New York, 1965. 1072 p. https://doi.org/10.1016/C2013-0-12490-7
9. Belozerova N. S., Zubo Y. O., Shugaev A. G., Kuznetsov V. V. Method for studying of individual mitochondrial gene transcription in plants. Vestnik RUDN, seriya Agronomiya i zhivotnovodstvo = RUDN Journal of Agronomy and Animal Industries, 2009, no. 1, pp. 65–72 (in Russian).
10. Lemeza N. A. Algology and mycology. Workshop. Minsk, Vysheishaya shkola Publ., 2008. 200 p. (in Russian).
11. Kirschenbaum D. M. Molar absorptivity and A1%1cm values for proteins at selected wavelengths of the ultraviolet and visible region. ХI. Analytical Biochemistry, 1975, vol. 68, no. 2, pp. 465–484. https://doi.org/10.1016/0003-2697(75)90642-9
12. Nikandrov V. N., Pyzhova N. S. Methods for the study of proteolysis. Sovremennye problemy biokhimii. Metody issledovanii [Modern problems of biochemistry. Research methods]. Minsk, 2013, pp. 132–157 (in Russian).
13. Nikandrov V. N., Pyzhova N. S., Votyakov V. I. Effect of organic solvents on the streptokinase initiated fibrinolysis. Voprosy medicinskoi khimii [Questions of Medical Chemistry], 1987, vol. 33, no. 1, pp. 84–87 (in Russian).
14. Nikandrov V. N., Pyzhova N. S. Regulatory proteins: functional properties of molecules and mechanisms of their biological action. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya biyalagichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Biological series, 2003, no. 3, pp. 75–89 (in Russian).
15. Pyzhova N. S., Nikandrov V. N. The effects of biogenic phosphates on proteinase-induced protein cleavage and functioning of plasminogen activator. Russian Journal of Bioorganic Chemistry, 2008, vol. 34, no. 3, pp. 344–352. https://doi.org/10.1134/s1068162008030163
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