Territorial distribution alleles of SSR-loci of chloroplast DNA of scots pine (Pinus sylvestris L.) in Belarus

Scotch pine (Pinus sylvestris L.) is one of the main forest forming species in Belarus, which cover 49.2 % of the country’s forested area. The aim of the study was to investigate spatial distribution of microsatellite (SSR) alleles (chloroplast DNA) and identify the features of the genetic structure and genogeographic differentiation of P. sylvestris populations in Belarus. Molecular genetic analysis of six SSR loci of Scotch pine cpDNA in samples form 73 naturally originated stands was carried out. 35 allelic variants of loci PCP1289, PCP4507, PCP83314, PCP71987, PCP26106, PCP30277 were identified. The analysis of the geographic distribution of the dominant allelic variants showed that the population structure of the pine forest is rather homogeneous. Certain regional differences in a number of cases were found for less common, but also widespread variants. One group of alleles is characterized by an increase or decrease in the frequency of occurrence in the direction from the southwest to the northeast. Another group includes allelic variants which share is maximal in the zone covering the Grodno region, the southwestern part of the Minsk region and the Gomel region, while to southwest and to northeast from this zone their frequency of occurrence decreases or is absent. A number of alleles were found only in one of the analyzed stands or in a limited area, which may indicate their local origin as a result of spontaneous mutations. The obtained results are important both from a general biological point of view in studying the evolution and formation of the genetic structure of P. sylvestris in Belarus, and from a practical point of view, since they allow to improve the forest seed zoning of the species.

Scots pine, Pinus sylvestris, Pinetum, SSR-locus, microsatellite analysis, chloroplast DNA

1. Nejshtadt M. I. History of forests and paleogeography of the USSR in the Holocene. Moscow, 1957. 404 p. (in Russian).

2. Kind N. V. Paleoclimates and the natural environment of the Holocene. Istoriya biogeocenozov SSSR v golocene [History of biogeocenoses of the USSR in the Holocene]. Moscow, 1976, pp. 5–14 (in Russian).

3. Zubakov V. A. Global climatic events of the Pleistocene. Leningrad, 1986. 288 p. (in Russian).

4. Zernickaya V. P. Reconstruction of human economic activity in the Holocene. Nauka i innovacii = Science and Innovations, 2011, no. 9 (103), pp. 16–19 (in Russian).

5. Hotinskij N. A. Holocene of Northern Eurasia. Moscow, 1977. 200 p. (in Russian).

6. Abramson N. I. Phylogeography: results, issues and perspectives. Informatsionnyi Vestnik VOGiS = Information Bulletin VOGiS, 2007, vol. 11, no. 2, pp. 307–331 (in Russian).

7. Sinclair W. T., Morman J. D., Ennos R. A. Multiple origins for Scots pine (Pinus sylvestris L.) in Scotland: evidence from mitochondrial DNA variation. Heredity, 1998, vol.809, no. 2, pp. 233–240. https://doi.org/10.1046/j.1365-2540.1998.00287.x

8. Soranzo N., Alia R., Provan J., Powell W. Patterns of variation at a mitochondrial sequence-tagged-site locus provides new insights into the postglacial history of European Pinus sylvestris populations. Molecular Ecology, 2000, vol. 9, no. 9, pp. 1205–1211. https://doi.org/10.1046/j.1365-294x.2000.00994.x

9. Gernandt D. S., López G. G., García S. O., Liston A. Phylogeny and classification of Pinus. Taxon, 2005, vol. 54, no. 1, pp. 29–42. https://doi.org/10.2307/25065300

10. Cheddadi R., Vendramin G. G., Litt T., François L., Kageyama M., Lorentz S., Laurent J.-M., de Beaulieu J.-L., Sadori L., Jost A., Lunt D. Imprints of Glacial Refugia in the Modern Genetic Diversity of Pinus sylvestris. Global Ecology and Biogeography, 2006, vol. 15, no. 3, pp. 271–282. https://doi.org/10.1111/j.1466-822x.2006.00226.x

11. Pyhäjärvi T., Salmela M. J., Savolainen O. Colonization Routes of Pinus sylvestris Inferred from Distribution of Mitochondrial DNA Variation. Tree Genetics and Genomes, 2008, vol. 4, no. 2, pp. 247–254. https://doi.org/10.1007/s11295- 007-0105-1

12. Hernandez-Leon S., Gernandt D. S., Rosa J. A., Jardon-Barbolla L. Phylogenetic Relationships and Species Delimitation in Pinus Section Trifoliae Inferrred from Plastid DNA. PLOS ONE, 2013, vol. 8, no. 7, art. e70501. https://doi. org/10.1371/journal.pone.0070501

13. Tong Y. W., Lewis B. J., Zhou W. M., Mao C. R., Wang Y., Zhou L., Yu D. P., Dai L. M., Qi L. Genetic Diversity and Population Structure of Natural Pinus koraiensis Populations. Forests, 2020, vol. 11, no. 1, pp. 39. https://doi.org/10.3390/ f11010039

14. Maniatis T., Sambrook J., Fritsch E. F. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Laboratory Press Publ., 1989. 324 p.

15. Padutov V. E., Baranov O. Yu., Voropaev E. V. Methods of molecular genetic analysis. Minsk, 2007. 176 p. (in Russian).