New immunoassay systems based on recombinant human lactoferrin
https://doi.org/10.29235/1561-8323-2021-65-3-290-302
Abstract
In this work, soluble and solid phase immunoreagents, including recombinant human lactoferrin (rhLF), a complex of rhLF with europium ions, rabbit antiserum to rhLF, anti-rhLF immunoglobulin purified by antigen-affinity chromatography and the conjugates of this immunoglobulin with an Eu3+ chelate or horseradish peroxidase have been obtained by a combination of biochemical and synthetic methods using rhLF as an initial compound. Biospecific interactions of the reagents in four immunochemical systems were assessed by measuring the enzyme activity or time-resolved fluorescence. The study resulted in the development of fast and precise immunoassays for biologically active rhLF in transgenic goat milk and in protein fractions obtained in the course of pure rhLF manufacture, as well as in pharmaceutical preparations and food additives.
About the Authors
D. A. Semenov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Semenov Dmitry A. – Researcher
5/2, Kuprevich Str., 220141, Minsk, Republic of Belarus
I. I. Vashkevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Vashkevich Irina I. – Ph. D. (Chemistry), Leading researcher
5/2, Kuprevich Str., 220141, Minsk, Republic of Belarus
O. V. Sviridov
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Sviridov Oleg V. – D. Sc. (Chemistry), Head of the Laboratory
5/2, Kuprevich Str., 220141, Minsk, Republic of Belarus
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