Determining the human chronological age from the blood samples on the basis of the analysis of CpG-dinucleotides methylation
https://doi.org/10.29235/1561-8323-2021-65-5-582-591
Abstract
Based on the bioinformatic and statistical analysis of the GEO-projects to determine the genome-wide profile of human DNA methylation, a list of 27 CpG dinucleotides with a high predictive potential was formed to create models for prediction of the human age from blood samples. The methylation level was determined for 245 samples of individuals from the Republic of Belarus. The correlation coefficients R were calculated, and the mathematical models for determining the age of an individual were constructed. The average accuracy value of the age prediction from blood samples using 12 CpG-dinucleotides was 3.4 years (for men – 3.3, for women – 3.5). The results obtained will be used as a basis for development of calculators for predicting the age of an individual based on the biological traces for forensic experts.
About the Authors
V. A. Lemesh
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Lemesh Valentina A. – Ph. D. (Biology), Assistant professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
V. N. Kipen
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Kipen Viachaslau N. – Ph. D. (Biology), Leading Researcher
27, Akademicheskaya Str., 220072, Minsk
M. V. Bahdanava
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Bahdanava Marina V. – Ph. D. (Biology), Leading researcher
27, Akademicheskaya Str., 220072, Minsk
A. A. Burakova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Burakova Arina A. – Junior researcher
27, Akademicheskaya Str., 220072, Minsk
A. A. Bulgak
Scientific and Practical Centre «Cardiology»
Belarus
Belarus
Bulgak Alexander G. – Corresponding Member, D. Sc.
(Medicine), Professor, Chief researcher
110B, Roza Lyuksemburg Str., 220036, Minsk
A. V. Bayda
Белорусская медицинская академия последипломного образования
Belarus
Belarus
Bayda Alexander V. – Ph. D. (Medicine), Head of the Laboratory
3, P. Brovka Str., 220013, Minsk
V. V. Zotova
Scientific and Practical Centre «Cardiology»
Belarus
Belarus
Zotova Volga V. – Ph. D. (Medicine), Cardiologist, Researcher
110B, Roza Lyuksemburg Str., 220036, Minsk
V. I. Dobysh
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Dobysh Volga I. – Junior researcher
27, Akademicheskaya Str., 220072, Minsk
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