Some metal binding properties of recombinant human lactoferrin from the milk of transgenic goats
https://doi.org/10.29235/1561-8323-2022-66-1-43-54
Abstract
In this research, the ability of pure recombinant human lactoferrin (rhLF), originated from the milk of transgenic goats, to bind ferric and europium ions has been shown by the methods of spectrophotometry, fluorescent spectroscopy, and inductively coupled plasma mass spectrometry. The apo-form of rhLF and its complexes with Fe3+ or Eu3+ saturated in 76 or 22 %, respectively, were obtained. A method for detection of total or released (“free”) lanthanide at acidic or neutral pH and high or low concentrations of chelating agents by time-resolved fluorescence was proposed.
About the Authors
D. A. Semenov
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Belarus
Belarus
Semenov Dmitry A. – Researcher.
5/2, Kuprevich Str., 220141, Minsk
O. S. Kuprienko
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Belarus
Belarus
Kuprienko Olga S. – Ph. D. (Chemistry), Senior Researcher.
5/2, Kuprevich Str., 220141, Minsk
I. I. Vashkevich
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Belarus
Belarus
Vashkevich Irina I. – Ph. D. (Chemistry), Leading Researcher.
5/2, Kuprevich Str., 220141, Minsk
O. V. Sviridov
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Belarus
Belarus
Sviridov Oleg V. – D. Sc. (Chemistry), Head of the Laboratory.
5/2, Kuprevich Str., 220141, Minsk
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