Productivity and grain nutritional value traits in wheat genotypes with different NAM-B1 gene allelic variations

The identification of a functional NAM-B1 allele associated with a high content of grain protein and essential microelements in wheat relatives increased the distant hybridization significance for bread wheat nutritional value. The allelic polymorphism of the NAM-B1 gene in 22 wheat lines with a genetic material of T. dicoccoides, T. dicoccum, T. spelta, T. kiharаe and their parental forms and the effects of NAM-B1 gene allelic variations on the content of grain protein and essential microelements and productivity traits (vegetation period 2017–2021) were evaluated. The functional NAM-B1 allele was identified only in the samples of wheat relatives among the parental forms. All parental varieties and most of introgressive lines (77.3 %) had a non-functional allele. The genotypes with the functional NAM-B1 allele were characterized by a higher plant height and tillering, but by lower spike productivity compared to the non-functional allele genotypes. The presence of the functional NAM-B1 allele provided a high level of grain protein and zinc content and never decreased significantly a thousand-kernel weight across all studied environments. The functional NAM-B1 allele introgression could be a resource for improving the grain wheat nutritional value.

1. Gupta P. K., Balyan H. S., Sharma S., Kumar R. Biofortification and bioavailability of Zn, Fe and Se in wheat: present status and future prospects. Theoretical and Applied Genetics, 2021, vol. 134, no. 1, pp. 1–35. https://doi.org/10.1007/s00122- 020-03709-7

2. Brevis J. C., Dubcovsky J. Effects of the chromosome region including the Gpc-B1 locus on wheat grain and protein yield. Crop Science, 2010, vol. 50, no. 1, pp. 93–104. https://doi.org/10.2135/cropsci2009.02.0057

3. Uauy C., Distelfeld A., Fahima T., Blechl A., Dubcovsky J. A NAC gene regulating senescence improves grain protein, zink, and iron content in wheat. Science, 2006, vol. 314, no. 5803, pp. 1298–1301. https://doi.org/10.1126/science.1133649

4. Waters B. M., Uauy C., Dubcovsky J., Grusak M. A. Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. Journal of Experimental Botany, 2009, vol. 60, no. 15, pp. 4263–4274. https://doi.org/10.1093/jxb/erp257

5. Yang R., Juhasz A., Zhang Y., Chen X., Zhang Y., She M., Zhang J., Maddern R., Edwards I., Diepeveen D., Islam S., Ma W. Molecular characterisation of the NAM-1 genes in bread wheat in Australia. Crop and Pasture Science, 2018, vol. 69, no. 12, pp. 1173–1181. https://doi.org/10.1071/cp18273

6. Ahmed H. G. M., Sajjad M., Zeng Y., Iqbal M., Khan S. H., Ullah A., Akhtar M. N. Genome-wide association mapping through 90K SNP array for quality and yield attributes in bread wheat against water-deficit conditions. Agriculture, 2020, vol. 10, no. 9, pp. 392. https://doi.org/10.3390/agriculture10090392

7. Kronenberg L., Yates S., Boer M. P., Kirchgessner N., Walter A., Hund A. Temperature response of wheat affects final height and the timing of stem elongation under field conditions. Journal of Experimental Botany, 2021, vol. 72, no. 2, pp. 700–717. https://doi.org/10.1093/jxb/eraa471

8. Carter A. H., Santra D. K., Kidwell K. K. Assessment of the effects of the Gpc-B1 allele on senescence rate, grain protein concentration and mineral content in hard red spring wheat (Triticum aestivum L.) from the Pacific Northwest Region of the USA. Plant Breeding, 2012, vol. 131, no. 1, pp. 62–68. https://doi.org/10.1111/j.1439-0523.2011.01900.x

9. Tabbita F., Lewis S., Vouilloz J. P., Ortega M. A., Kade M., Abbate P. E., Barneix A. J. Effects of the Gpc-B1 locus on high grain protein content introgressed into Argentinean wheat germplasm. Plant Breeding, 2013, vol. 132, no. 1, pp. 48–52. https:// doi.org/10.1111/pbr.12011

10. Vishwakarma M. K., Arun B., Mishra V. K., Yadav P. S., Kumar H., Joshi A. K. Marker-assisted improvement of grain protein content and grain weight in Indian bread wheat. Euphytica, 2016, vol. 208, no. 2, pp. 313–321. https://doi.org/10.1007/ s10681-015-1598-6

11. Tabbita F., Pearce S., Barneix А. J. Breeding for increased grain protein and micronutrient content in wheat: ten years of the GPC-B1 gene. Journal of Cereal Science, 2017, vol. 73, pp. 183–191. https://doi.org/10.1016/jcs.2017.01.003

12. Zhao F. J., Su Y. H., Dunham S. J., Rakszegi M., Bedo Z., McGrath S. P., Shewry P. R. Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. Journal of Cereal Science, 2009, vol. 49, no. 2, pp. 290–295. https://doi.org/10.1016/j.jcs.2008.11.007

13. Orlovskaya O. A., Vakula S. I., Khotyleva L. V., Kilchevsky A. V. Correlations and variation structure of grain quality traits and yield of wheat lines with foreign genetic material. Molekulyarnaya i prikladnaya genetika = Molecular and Applied Genetics, 2021, vol. 31, pp. 42–52 (in Russian). https://doi.org/10.47612/1999-9127-2021-31

14. Pearce S., Tabbita F., Cantu D., Buffalo V., Avni R., Vazquez-Gross H., Zhao R., Conley C. J., Distelfeld A., Dubcovksy J. Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence. BMC Plant Biol, 2014, vol. 14, no. 1, p. 368. https://doi.org/10.1186/s12870-014-0368-2