Kinetics of fibrin clots destruction under ultrasonic cavitation
https://doi.org/10.29235/1561-8323-2022-66-6-587-594
Abstract
We studied the kinetic features of fibrin clot destruction in vitro under the action of ultrasonic cavitation generated by low-frequency (36 kHz) ultrasound (US) with the intensity I0 of 4.4–51.2 W/cm2, using a flexible waveguide concentrator. It was established that the rate of US destruction of clots immersed in saline at the initial stage of the process is proportional to I0 in the range of 12–51 W/cm2, corresponds to first-order kinetics, and is determined by the erosive processes without the formation of D-dimers and other fibrinolysis products at a minimum contribution of sonochemical reactions. The clot destruction rate is maximum at the initial time moment and decreases with increasing the US exposure duration (by 35 % in 1 min and by 72 % by the end of the second minute at I0 = 51.2 W/cm2). It was shown that in order to increase the completeness of clot destruction at a minimum administered US dose, it is advisable to minimize the US exposure time when using the highest values of the US intensity limited by the level of safe cavitation exposure to the vascular wall, hemostasis, and blood cells.
Keywords
fibrin clot,
kinetics,
thrombolysis,
acoustic cavitation,
stable and inertial (transient) cavitation
Supporting agencies: The work was supported by the Belarusian Republican Foundation for Fundamental Research (grant M21-004).
About the Authors
I. E. Adzerikho
Belarusian Medical Academy of Postgraduate Education
Belarus
Belarus
Igor E. Adzerikho – D. Sc. (Medicine), Professor. Belarusian Medical Academy of Postgraduate Education.
3/3, P. Brovka Str., 220013, Minsk
A. I. Kulak
Institute of General and Inorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Anatoly I. Kulak – Academician, D. Sc. (Chemistry), Professor, Director. Institute of General and Inorganic Chemistry of the National Academy of Sciences of Belarus.
9/1, Surganov Str., 220072, Minsk
S. M. Rachok
Belarusian Medical Academy of Postgraduate Education
Belarus
Belarus
Svetlana M. Rachok – Ph. D. (Medicine), Associate Professor. Belarusian Medical Academy of Postgraduate Education.
3/3, P. Brovka Str., 220013, Minsk
V. T. Minchenya
Belarus Scientific and Technological Park of the BNTU “Polytechnic”
Belarus
Belarus
Vladimir T. Minchenya – Ph. D. (Engineering), Associate Professor. Science and Technology Park BNTU “Polytechnic”.
24, Ya. Kolas Str., 220013, Minsk
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