Protective effect of quercetin encapsulated in gelatin nanoparticles against induced cellular oxidative stress
https://doi.org/10.29235/1561-8323-2026-70-1-45-53
Abstract
The aim of this study was to evaluate the potential of gelatin nanoparticles as a means of increasing the efficacy and bioavailability of phytocompounds, in particular quercetin. The protective effect of native quercetin and quercetin incorporated into gelatin nanoparticles was studied by initiating oxidative stress in human keratinocytes with tert-butyl hydroperoxide. Cell viability was assessed using the PrestoBlueTM reagent, apoptotic and necrotic cells were detected by double intravital staining using an assay kit including annexin V-FITC. DNA damage was analyzed using the comet assay. The results demonstrated that encapsulation of quercetin into gelatin nanoparticles enables its application in aqueous suspensions without compromising its antioxidant capacity, geneand cytoprotective effects under cellular oxidative stress conditions, indicating a high efficiency of quercetin release from this nanocarrier. Consequently, the utilization of gelatin nanoparticles represents a promising approach for improving the bioavailability and therapeutic efficacy of phytochemicals.
Keywords
apoptosis,
keratinocytes,
tert-butyl hydroperoxide,
quercetin,
nanostructures,
oxidative stress,
comet assay
Supporting agencies: This work was completed as part of task 3.03.11 of the State Program for Scientific Research “Convergence 2025”
About the Authors
A. I. Potapovich
Belarusian State University
Belarus
Belarus
Potapovich Alla I. – Ph. D. (Biology), Associate Professor, Leading Researcher.
4, Nezavisimosti Ave., 220030, Minsk
T. V. Kostyuk
Belarusian State University
Belarus
Belarus
Kostyuk Tatyana V. – Researcher.
4, Nezavisimosti Ave., 220030, Minsk
T. G. Shutova
Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus
Belarus
Belarus
Shutava Tatsiana G. – Ph. D. (Chemistry), Leading Researcher.
36, F. Skorinа Str., 220084, Minsk
V. A. Kostyuk
Belarusian State University
Russian Federation
Russian Federation
Kostyuk Vladimir A. – D. Sc. (Chemistry), Professor, Head of the Laboratory.
4, Nezavisimosti Ave., 220030, Minsk
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