ENZYMATIC ACTIVITY OF ARTHROBAC TER GENUS BACTERIA AND THEIR GENOTYPIC IDENTIFICATION
Abstract
Two-stage screening enabled one to define the composition of enzyme complexes produced by bacteria of genus Arthrobacter in media with specific substrates and activity of individual constituents involved in the t ransformation of milk proteins, lipids and carbohydrates. It was found that enzyme complexes of Arthrobacter strains BIM B-2239, BIM B-2240, BIM B-2241 and BIM B-2242 showed the most balanced activity of lipase, protease, β-galactosidase and glucose(xylose)isomerase components. Following the nucleotide sequence analysis of 16S rRNA gene the examined cultures were identified as Arthrobacter sulfonivorans. 16S rRNA gene sequences over 1400 bp in size were deposited in GenBank database.
Detailed investigation of conditions favoring the efficient hydrolysis of proteins and lipids by proteases and lipases produced by Arthrobacter sulfonivorans strains BIM B-2239, BIM B-2240, BIM B-2241, BIM B-2242 and the transformation of milk lactose and derived hydrolysis products mediated by β-galactosidases and glucose(xylose)isomerases will allow one to select microbial strain to develop the biotechnology of manufacturing hypoallergenic feed additive with prebiotic activity from dairy substrates.
About the Authors
L. I. SAPUNOVA
Институт микробиологии НАН Беларуси, Минск
Belarus
Belarus
A. A. KASTSIANEVICH
Институт микробиологии НАН Беларуси, Минск
Belarus
Belarus
A. G. LOBANOK
Институт микробиологии НАН Беларуси, Минск
Belarus
Belarus
I. O. TAMKOVICH
Институт микробиологии НАН Беларуси, Минск
Belarus
Belarus
S. A. KULISH
Институт микробиологии НАН Беларуси, Минск
Belarus
Belarus
D. P. BAZHANAU
Институт генетики и цитологии НАН Беларуси, Минск
Belarus
Belarus
K. K. YATSEVICH
Институт генетики и цитологии НАН Беларуси, Минск
Belarus
Belarus
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