FEATURES OF THE PROTEIN-PROTEIN INTERACTION OF CYTOCHROME P450 AND PLА2 OF DIFFERENT NATURE REVEALED USING CIRCULAR DICHROISM SPECTROSCOPY
Abstract
The study of the features of the protein-protein interaction of cytochrome P450 (CYP3A4 and CYP2B4) and phospholipase A2 (PLA2) using circular dichroism spectroscopy was carried out.
The divergent effects were found in the change of the CD-spectrum of the mixture of snake venom PLA2–CYP2B4 (ratio 1 : 4 mole/mole) and the mixture of porcine pancreatic PLA2-human CYP3A4 (ratio 1 : 20 mole/mole), as compared to the CD-spectra of the individual protein, respectively, which evidences direct protein-protein interactions of PLA2 and CYP.
The resultant CD-spectrum in the first case is characterized by a 2.5-fold increase in the molar ellipticity protein mixture in relation to the molar ellipticity of cytochrome P450, and the second – by a significant reduction of this index, which reflects the significant changes in the conformation of the interaction of these biopolymers.
The divergent effects were found in the change of the CD-spectrum of the mixture of snake venom PLA2–CYP2B4 (ratio 1 : 4 mole/mole) and the mixture of porcine pancreatic PLA2-human CYP3A4 (ratio 1 : 20 mole/mole), as compared to the CD-spectra of the individual protein, respectively, which evidences direct protein-protein interactions of PLA2 and CYP.
The resultant CD-spectrum in the first case is characterized by a 2.5-fold increase in the molar ellipticity protein mixture in relation to the molar ellipticity of cytochrome P450, and the second – by a significant reduction of this index, which reflects the significant changes in the conformation of the interaction of these biopolymers.
About the Authors
N. M. Litvinko
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Russian Federation
D. Sc. (Chemistry), Assistant Professor, Head of the Laboratory
Russian Federation
D. Sc. (Chemistry), Assistant Professor, Head of the Laboratory
G. N. Antonchik
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Russian Federation
Ph. D. (Chemistry), Researcher
Russian Federation
Ph. D. (Chemistry), Researcher
T. G. Glushakova
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Russian Federation
Russian Federation
D. O. Gerlovsky
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Russian Federation
Ph. D. (Chemistry), Researcher
Russian Federation
Ph. D. (Chemistry), Researcher
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