OXIDATIVE STRESS AS ONE OF THE POSSIBLE WAYS OF ANTICANCER EFFECTS OF BRASSINOSTEROIDS
Abstract
In this paper, the effect of brassinosteroids – 24-epibrassinolide and 28-homocastasterone, as well as their stereoisomers – (22S,23S)-24-homobrassinolide and (22S,23S)-28-homocastasterone on the level of reactive oxygen species in cancer cell line A549 (lung adenocarcinoma) has been characterized for the first time, and the results obtained have been compared with the data on their antiproliferative activity.
The effect depended on the structure of the side chain and was more pronounced in the case of the SS-orientation of hydroxyl groups at the C22 and C23 positions ((22S,23S)-28-homocastasterone). This compound also had greater antiproliferative activity, which suggested a possible relationship between the induction of activated oxygen species and cytotoxicity of the substances.
The results of this work allow one to assume that one of the ways of the effect of brassinosteroids on cancer cells may lie in the initiation of oxidative stress, which leads to the death of cancer cells through ROS-dependent mechanisms.
About the Authors
P. A. KISELEVBelarus
O. V. PANIBRAT
Belarus
A. G. SYSA
Belarus
M. V. ANISOVICH
Belarus
V. N. ZHABINSKII
Belarus
V. A. KHRIPACH
Belarus
References
1. WHO Cancer: Factsheet N. 297, February 2012.
2. Siegel, R. Cancer statistics / R. Siegel, D. Naishadham, A. Jemal // Cancer J. Clin. – 2012. – Vol. 62(1). – P. 10–29.
3. Cardiac side-effects of cancer chemotherapy / J. J. Monsuez [et al.] // Int. J. Cardiol. 2010. Vol. 144. P. 3–15.
4. Cell Death Pathways in Photodynamic Therapy of Cancer / P. Mroz [et al.] // Cancers. – 2011. – Vol. 3. – P. 2516–2539.
5. Khripach, V. A. Brassinosteroids. A new class of plant hormones / V. A. Khripach, V. N. Zhabinskii, A. de Groot. – San Diego: Academic Press, 1999.
6. Zhabinskii, N. Steroid plant hormones: Effects outside plant kingdom /N. Zhabinskii, N. Khripach, V. Khripach // Steroids. – 2015. – Vol. 97. – P. 87–97.
7. Anticancer and antiproliferative activity of natural brassinosteroids / J. Malikova [et al.] // Phytochemistry. – 2008. – Vol. 69. – P. 418–426.
8. Toxicity of (22R, 23R)-22,23-dihydroxystigmastane derivatives to cultured cancer cells / A. Misharina [et al.] // Steroids. – 2010. – Vol. 75. – P. 287–294.
9. Anticancer Activities of Brassinosteroids / L. Hoffmannová [et al.] / Brassinosteroids: Practical Applications in Agriculture and Human Health. – 2012. – P. 84–93.
10. Взаимосвязь структура-функция при оценке антипролиферативной активности брассиностероидов в отношении раковых клеток молочной железы MCF-7 / A. G. Sysa [et al.] // Vestnik Found Fund Res. – 2011. – Vol. 5. – P. 56–63.
11. Van Meerloo, J. Cell Sensitivity Assays: The MTT Assay / J. Van Meerloo, G. J. Kaspers, J. Cloos // Methods Mol. Biol. – 2011. – Vol. 731. – P. 237–245.
12. Новый синтез (22S,23S)-гомобрассинолида / А. А. Ахрем [и др.] // Докл. Академии наук СССР. – 1985. – Т. 283. – С. 130–133.
13. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induced cytochrome P450s alter the formation of reactive oxygen species in liver cells / S. Knerr [et al.] // Mol. Nutr. Food Res. – 2006. – Vol. 50. – P. 378–384.
14. Eruslanov, E. Identification of ROS using oxidized DCFDA and flow-cytometry / E. Eruslanov, S. Kusmartsev // Methods Mol. Biol. – 2010. – Vol. 594. – P. 57–72.
15. Reactive Oxygen Species in Vascular Formation and Development / Y. Zhou [et al.] // Oxid. Med. Cell Longev. – 2013. – Vol. 2. – P. 10–25.