BIOPOLYMER MULTILAYER FILMS AS SUBSTRATES FOR MESENCHYMAL STEM CELLS

Multilayer films (chitosan/dextran)_n , (chitosan/pectin)_n , (chitosan/carboxymethylcellulose)_n , (polyethyleneimine/dextran)_n , (polyethyleneimine/pectin)_n , (polyethyleneimine/carboxymethylcellulose)_n (n = 4; 4.5) with a thickness of 8.5 to 396.3 nm have been obtained. The relationship between the physicochemical characteristics of the films and the adhesion of mesenchymal stem cells to them has been determined. It has been shown that the mesenchymal stem cells effectively adhere to defect-free ultrathin multilayer viscoelastic chitosan-containing films and form on their surface a monolayer culture of fibroblast-like cells with high viability. 

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