PHOSPHOLIPASE A2 IB – A NEW INDICATOR FOR ESTIMATION OF THE PRO-ANTIOXIDANT STATUS OF THE ORGANISM
Abstract
The efficiency of free radical oxidation of phospholipid membranes under exposure to UV radiation (λ = 180– 400 nm) by the phospholipase A2 PLA2 IB as a marker of lipid peroxidation was studied. Mixed micelles of phosphatidylcholine and sodium deoxycholate were employed as the model of phospholipid membranes. The PLA2 activity was determined by spectral changes in hemoglobin upon the interaction with fatty acids, resulting from a phospholipolysis product. At the same time, the level of lipid peroxidation was estimated by accumulation of malondialdehyde as a well-known product of irradiation of phospholipids. The directly proportional dependence of the PLA2 activity on the UV irradiation time of phospholipids is obtained. It is shown that the level of the PLA2 activity correlates well with the lipid peroxidation level, pointing to the antioxidant capacity of blood in comparison with the well-known antioxidant Trolox as standard.
About the Authors
N. M. Litvinko
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
D. Sc. (Chemistry), Assistant Professor, Head of the Laboratory
L. A. Skorostetskaya
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Researcher
D. O. Gerlovsky
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Chemistry), Researcher
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