COMPARATIVE KINETIC CHARACTERISTIC OF THE ARTHROBACTER SULFONIVORANS STRAIN GROWTH AND THE EXTRACELLULAR BETA-GALACTOSIDASE SYNTHESIS
Abstract
The data are presented on kinetic correlations of the growth of parent and lactose-adapted strains of bacteria Arthrobacter sulfonivorans and the production of extracellular beta-galactosidase. It was found that the adapted strain BIM B-499-D was distinguished by a shorter lag phase than the parent strain BIM B-2242 by a reduced period of reaching a maximum specific growth rate (μmax = 0.316–0.319 h–1) and a stationary phase of culture. Synthesis of extracellular enzyme in both strains occurred during the exponential growth phase and attained a peak specific rate (εmax = 0.247–0.250 U · mg–1 · h–1) with a 6 h time interval. BIM B-499-D exceeds BIM B-22421.6 times in the level of enzyme biosynthesis and in the duration of the process – 1.3 times. The kinetic parameters of growth (tµmax = 6–9 h) and beta-galactosidase production (tεmax = 18–24 h) established for the examined strains indicate the disconnection of processes in time at least by 12–15 h.
About the Authors
L. I. Sapunova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Biology), Chief researcher
A. G. Lobanok
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Academician, D. Sc. (Biology), Head of the Laboratory
I. A. Tamkovich
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Biology), Senior researcher
A. A. Kastsianevich
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Biology), Head of the Laboratory
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