DIPLOID HYBRIDS BETWEEN THE WILD ALLOTETRAPLOID POTATO SPECIES SOLANUM STOLONIFERUM SCHLDTL & BOUCHET AND DIPLOID CLONES OF CULTIVATED POTATO S. TUBEROSUM L. HAVING GENOME В OF WILD SPECIES

We were the first who demonstrated the possibility to produce diploid hybrids (along with triploid ones) while pollinating some wild allotetraploid potato species with the pollen of S. tuberosum diploid clones. It was determined that these hybrids only retained one of the genomes of wild species (genome A). Here, we present the results of revealing diploid interspecific hybrids having genome B of wild species. There were selected four hybrids producing the viable pollen among 235 hybrids between valuable for breeding accessions of S. stoloniferum and fertile diploid clone of S. tuberosum. The analysis of meiosis in pollen mother cells showed that they had 24chromosomes. The DNA marker SolB of genome B of wild species was detected in diploid hybrids, as well as in parental clones of S. stoloniferum and triploid interspecific hybrids. The diploid hybrids inherited the typical for wild species markers RYSC 3 and Yes3-3A of Y-virus resistance genes, as well as the markers of late blight resistance genes R1 and R3b. Two of the diploid hybrids inherited markers of late blight resistance gene Rpi-sto1 presented in parental clones of the wild species lossless. The marker 517/1519 of this gene was not detected in one diploid hybrid and both markers (517/1519 and 1521/518) − in another one. Production of the hybrids with genome B makes the version on a possible mechanism of their formation through the selective elimination of one of the genomes of wild species in ontogenesis of initially triploid interspecific hybrids to be priority. 

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