SYNTHESIS OF 24-EPICASTASTERONE CONJUGATE WITH FLUORESCENT DYE BODIPY
https://doi.org/10.29235/1561-8323-2018-62-2-178-184
Abstract
To carry out studies of molecular events involving brassinosteroids in a living cell, the synthesis of the phytohormone 24-epicastasterone labeled with a fluorescent dye BODIPY was performed. A distinctive feature of the conjugate obtained is the preservation in its molecule of native functional groups of brassinosteroids responsible for the physiological effects of this class of compounds, which is intended to ensure its effective binding to the corresponding receptor. The reaction of azide-alkyne [3 + 2]-cycloaddition of steroid azide with an acetylene BODIPY-containing dye fragment was used as a key stage in chemical synthesis.
About the Authors
A. L. Hurski
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
A. V. Baranovsky
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
P. Drasar
Университет химии и технологии, Прага
Czech Republic
Czech Republic
V. N. Zhabinskii
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
V. A. Khripach
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, Minsk
Belarus
Belarus
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