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THE ROLE OF RLM AND AVRLM GENES IN THE REALIZATION OF RAPE SPECIFIC RESISTANCE TO BLACKLEG

https://doi.org/10.29235/1561-8323-2018-62-2-203-209

Abstract

As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.

About the Authors

V. A. Lemesh
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Ph. D. (Biology), Assistant professor, Director


G. V. Mozgova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Ph. D. (Biology), Head of the Centre


Ya. E. Pilyuk
Research and Practical Center of the National Academy of Sciences of Belarus for Arable Farming, Zhodino
Belarus
Ph. D. (Agrarian), Assistant professor, Head of the Department


N. E. Horuzhiy
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Junior researcher


E. S. Byk
Research and Practical Center of the National Academy of Sciences of Belarus for Arable Farming, Zhodino
Belarus
Researcher


A. A. Burakova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Minsk
Belarus
Junior researcher


References

1. Fitt B. D. H., Brun H., Barbetti M. J., Rimmer S. R. World-wild importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). European Journal of Plant Pathology, 2006, vol. 114, no. 1, pp. 3–15. DOI: 10.1007/s10658-005-2233-5

2. Raman R., Taylor B., Marcroft S., Stiller J., Eckermann P., Coombes N., Rehman A., Lindbeck K., Luckett D., Wratten N., Batley J., Edwards D., Wang X., Raman H. Molecular mapping of qualitative and quantitative loci for resistance to Leptosphaeria maculans causing blackleg disease in canola (Brassica napus L.). Theoretical and Applied Genetics, 2012, vol. 125, no. 2, pp. 405–418. DOI: 10.1007/s00122-012-1842-6

3. Delourme R., Chèvre A. M., Brun H., Rouxel T., Balesdent M. H., Dias J. S., Salisbury P., Renard M., Rimmer S. R. Major gene and polygenic resistance to Leptosphaeria maculans in oilseed rape (Brassica napus). European Journal of Plant Pathology, 2006, vol. 114, no. 1, pp. 41–52. DOI: 10.1007/s10658-005-2108-9

4. Dilmaghani A., Balesdent M. H., Didier J. P., Wu C., Davey J., Barbetti M. J., Li Hua, Moreno-Rico O., Phillips D., Despeghel J. P., Vincenot L., Gout L., Rouxel T. The Leptosphaeria maculans – Leptosphaeria biglobosa species complex in the American continent. Plant Pathology, 2009, vol. 58, no. 6, pp. 1044–1058. DOI: 10.1111/j.1365-3059.2009.02149.x

5. Ansan-Melayah D., Rouxel T., Bertrandy J., Letarnec B., Mendes-Pereira E., Balesdent M. H. Field efficiency of Brassica napus specific resistance correlates with Leptosphaeria maculans population structure. European Journal of Plant Pathology, 1997, vol. 103, no. 9, pp. 835–841. DOI: 10.1023/a:1008605829110

6. Balesdent M. H., Louvard K., Pinochet X., Rouxel T. A large-scale survey of races of Leptosphaeria maculans occurring in oilseed rape in France. European Journal of Plant Pathology, 2006, vol. 114, no. 1, pp. 53–65. DOI: 10.1007/s10658-005-2104-0

7. Rouxel T., Balesdent M. H. The stem canker (blackleg) fungus, Leptosphaeria maculans, enters the genomic era. Molecular Plant Pathology, 2005, vol. 6, no. 3, pp. 225–241. DOI: 10.1111/j.1364-3703.2005.00282.x

8. Rouxel T., Penaud A., Pinochet X., Brun H., Gout L., Delourme R., Schmit J., Balesdent M.-H. A ten-year survey of populations of Leptosphaeria maculans in France a rapid adaptation towards the Rlm1 resistance gene in oilseed rape. European Journal of Plant Pathology, 2003, vol. 109, no. 8, pp. 871–881. DOI: 10.1023/A:1026189225466

9. West J. S., Fitt B. D. L. Population dinamics and dispersal of Leptosphaeria maculans (blackleg of canola). Australasian Plant Pathology, 2005, vol. 34, no. 4, pp. 457–461. DOI: 10.1071/ap05086

10. Mendes-Pereira E., Balesdent M.-H., Brun H., Rouxel T. Molecular phylogeny of the Leptosphaeria maculans – L. biglobosa species complex. Mycological Research, 2003, vol. 107, no. 11, pp. 1287–1304. DOI: 10.1017/s0953756203008554

11. Gout L., Fudal I., Kuhn M.-L., Blaise F., Eckert M., Cattolico L., Balesdent M.-H., Rouxel T. Lost in the middle of nowhere: the AvrLm1 avirulence gene of the Dothideomycete Leptosphaeria maculans. Molecular Microbiology, 2006, vol. 60, no. 1, pp. 67–80. DOI: 10.1111/j.1365-2958.2006.05076.x

12. Fudal I., Ross S., Brun H., Besnard A.-L., Ermel M., Kuhn M.-L., Balesdent M.-H., Rouxel T. Repeat-induced point mutation (RIP) as an alternative mechanism of evolution towards virulence in Leptosphaeria maculans. Molecular PlantMicrobe Interactions, 2009, vol. 22, no. 8, pp. 932–941. DOI: 10.1094/mpmi-22-8-0932

13. Parlange F., Daverdin G., Fudal I., Kuhn M.-L., Balesdent M.-H., Blaise F., Grezes-Besset B., Rouxel T. Leptosphaeria maculans avirulence gene AvrLm4-7 confers a dual recognition specificity by the Rlm4 and Rlm7 resistance genes of oilseed rape, and circumvents Rlm4-mediated recognition through a single amino acid change. Molecular Microbiology, 2009, vol. 71, no. 4, pp. 851–863. DOI: 10.1111/j.1365-2958.2008.06547.x

14. Marcroft S. J., Elliott V. L., Cozijnsen A. J., Salisbury P. A., Howlett B. J., Van de Wouw A. P. Identifying resistance genes to Leptosphaeria maculans in Australian Brassica napus cultivars based on reactions to isolates with known avirulence genotypes. Crop and Pasture Science, 2012, vol. 63, no. 4, pp. 338–350. DOI: 10.1071/cp11341

15. Dusabenyagasani M., Fernando W. G. D. Development of a SCAR marker to track canola resistance against blackleg caused by Leptosphaeria maculans pathogenisity group 3. Plant Disease, 2008, vol. 92, no. 6, pp. 903–908. DOI: 10.1094/ pdis-92-6-0903


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ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)