Analysis of structural and functional organization of the curly birch chloroplast genome based on the next-generation sequencing data
https://doi.org/10.29235/1561-8323-2019-63-3-312-316
Abstract
The sequencing and annotation of the curly birch chloroplast genome were carried out. A high level of similarity of the structural and functional organization of cpDNA among the species of the Betulaceae family was revealed. A set of primers was developed to assess the level of expression of EST markers of the curly birch cpDNA by the real time PCR method.
Supporting agencies: The work was sponsored by the Belarusian Republication Foundation for Fundamental Research (Project No. Б17-63).
About the Authors
O. Yu. Baranov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Baranov Oleg Yur’evich – D. Sc. (Biology), Assistant professor, Head of the Department
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
P. S. Kiryanov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Kiryanov Pavel Sergeevich – Junior researcher
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
S. V. Pantelev
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Panteleev Stanislav Victorovich – Ph. D. (Biology), Senior researcher
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
L. V. Mozharovskaya
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Mozharovskaya Ludmila Valentinovna – Junior researcher
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
A. V. Padutov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Padutov Alexandr Vladimirovich – Junior researcher
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
O. A. Razumova
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Razumova Olga Alexandrovna – Ph. D. (Biology), Senior researcher
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
V. E. Padutov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Padutov Vladimir Evgen’evich – Corresponding Member, D. Sc. (Biology), Head of the Laboratory
71, Proletarskaya Str., 246050, Gomel, Republic of Belarus
References
1. Vetchinnikova L. V., Titov A. F. Genesis of the karelian Birch. An Ecogenetic hypothesis. Ecoligical genetics, 2016, vol. 14, no. 2, pp. 3–18 (in Russian). https://doi.org/10.17816/ecogen1423-18
2. Bajaj Y. S. Plant Protoplasts and Genetic Engineering II. Berlin, 1989. 499 p. https://doi.org/10.1007/978-3-642-74454-9
3. Jansen R. K., Raubeson L. A., Boore J. L., De Pamphilis C. W., Chumley T. W., Haberle R. C., Wyman S. K., Alverson A. J., Sallie R. P., Herman J., Matthew H., Jennifer F., Kuehl V., McNeal J. R., Leebens-Mack J., Cui L. Methods for obtaining and analyzing whole chloroplast genome sequences. Methods in enzymology, 2005, vol. 395, pp. 348–384. https://doi.org/10.1016/s0076-6879(05)95020-9
4. National center for biotechnology information. Available at: https://www.ncbi.nlm.nih.gov (аccessed 17 July 2018).
5. The list of PCR primers for amplification of the loci of cpDNA. Available at: https://mega.nz/#!WoUXSA7l!BQ3BtGtZAI7jnQsEX0Vh7VaXNHjU4lJPJD9oWRMU2-4 (аccessed 13 August 2018).
Review
Views: 929
Email this article 