Influence of sodium glutamate and food additives on the activity of smooth muscles of the stomach, the intestines and the cardiac activity of rats
https://doi.org/10.29235/1561-8323-2019-63-3-331-342
Abstract
The present work investigates the action of food additives: sodium glutamate E-621 (720 mg/kg), sodium benzoate E-211 (30 mg/kg), tartrazine E-102 (45 mg/kg) on the modulation of the heart rate (СR) and the activity of smooth muscles of the stomach and the colon in acute experiments on rats. Narcosis – thiopental sodium, 70 mg/kg is made intraperitoneally. It is established that the unitary administration of each of the additives into the stomach separately or everywhere results in precise effects of increase or decrease of total potentials of smooth muscles (PSM) without CR changes. After introducing glutamate into the stomach intravenously infusion adrenaline (Adr, 10 mkg) causes CR to increase more significantly, if all additives are introduced into the stomach, and then Adr. PSMs after the application of Adr were often more stimulating. Later, a subsequent significant influence on the effects of long use of additives in a diet is established. As it has appeared, the application of adrenaline in finishing acute experiment (after a month of feeding by additives) is accompanied not by amplification(strengthening), and, on the contrary, by the disorder of the heart work: CR gradually falls, arrhythmia arises, fibrillations of a myocardium and then cardiac contractions stopped in 12 of 16 experiments. These processes are associated with decreas ing the background activity on an electrocardiogram of the average area of RST (in the control 149 mkV/s, in a month of experience 80 mkV/s, р < 0.05) and waves Т (82 and 43 mkV/s, respectively, р < 0.01) without significant changes of their duration. The increase or decrease of the RST area is seen and Т is marked at the action of special ADR on the stomach additives. It is supposed that a long use of food additives serves as the precondition for dysfunctions in the heart work in the situations connected to a fast increase in the blood of catecholamines – in the conditions of various stresses, adverse influences of factors of the environment.
About the Authors
V. V. Soltanov
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Soltanov Vladimir Vsevolodovich – Corresponding Member, D. Sc. (Biology), Professor, Chief researcher
28, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
L. M. Komarovskaya
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Komarovskaya Ludmila Mikhailovna – Researcher
28, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
References
1. Kitamura A., Tsurugizawa T., Uematsu A., Torii K., Uneyama H. New therapeutic strategy for amino acid medicine: effects of dietary glutamate on gut and brain function. Journal Pharmacology Science, 2012, vol. 118, no. 2, pp. 138–144. https://doi.org/10.1254/jphs.11r06fm
2. Mazzoli R., Pessione E. The Neuroendocrinological Role of Microbial Glutamate and GABA Signaling. Frontiers in Microbiology, 2016, vol. 7, pp. 1934. https://doi.org/10.3389/fmicb.2016.01934
3. Azev O. A., Burko V. E., Soltanov V. V. Program product “Inputwin” for registration and analysis of the electrophysiological data. Novosti mediko-biologicheskikh nauk = News of Biomedical Sciences, 2010, vol. 2, no. 4, pp. 152–155 (in Russian).
4. Fukuda K., Kanazawa H., Aizawa Y., Ardell J. L., Shivkumar K. Cardiac innervation and sudden cardiac death. Circulation Research, 2015, vol. 116, no. 12, pp. 2005–2019. https://doi.org/10.1161/circresaha.116.304679
5. Japundžić-Žigon N., Šarenac O., Lozić M., Vasić M., Tasić T., Bajić D., Kanjuh V., Murphy D. Sudden death: Neurogenic causes, prediction, and prevention. European Journal of Preventive Cardiology, 2018, vol. 25, no. 1, pp. 29–39. https://doi.org/10.1177/2047487317736827
6. Philbin K. E., Bateman R. J., Mendelowitz D. Clonidin, an alpha2-receptor agonist, diminishes GABAergic neurotransmission to cardiac vagal neurons in the nucleus ambiguus. Brain Research, 2010, vol. 1347, pp. 65–70. https://doi.org/10.1016/j.brainres.2010.06.001
7. Shin E., Ko K. S., Rhee B. D., Han J., Kim N. Different effects of prolonged β-adrenergic stimulation on heart and cerebral artery. Integrative Medicine Research, 2014, vol. 3, no. 4, pp. 204–210. https://doi.org/10.1016/j.imr.2014.10.002
8. Garcia-Elias A., Benito B. Ion Channel Disorder and Sudden Cardiac Death. International Journal of Molecular Sciences, 2018, vol. 19, no. 3, pp. 692. https://doi.org/10.3390/ijms19030692
9. Ashton J. L., Burton R. A. B., Bub G., Smaill B. H., Montgomery J. M. Synaptic Plasticity in Cardiac Innervation and Its Potential Role in Atrial Fibrillation. Frontiers in Physiology, 2018, vol. 9, pp. 240. https://doi.org/10.3389/fphys.2018.00240
10. Ishibashi H., Umezu M., Jang I. S., Ito Y., Akaike N. Alpha1-adrenoreceptor-activated cation currents in neurones acutely isolated from rat cardiac parasympathetic ganglia. Journal of Physiology, 2003, vol. 548, no. 1, pp. 111–120. https://doi.org/10.1111/j.1469-7793.2003.00111.x
11. Soltanov V. V. Role of the gut microflora in the mechanisms for nervous regulation of autonomic functions. Novosti mediko-biologicheskikh nauk = News of Biomedical Sciences, 2015, vol. 11, no. 2, pp. 184–194 (in Russian).
12. Piper P. W. Yeast superoxide dismutase mutants reveal a pro-oxidant action of weak organic acid food preservatives. Free Radical Biology and Medicine, 1999, vol. 27, no. 11–12, pp. 1219–1227. https://doi.org/10.1016/s0891-5849(99)00147-1
13. Guimaraes P. S., Huber D. A., Compagnole-Santos M. J., Schreihofer A. M. Development of attenuated baroreflexes in obese Zucker rats coincides with impaired activation of nucleus tractus solitarius. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2014, vol. 306, no. 9, pp. R681–R692. https://doi.org/10.1152/ajpregu.00537.2013
14. Schultz H. D., Marcus N. J., del Rio R. Mechanisms of carotid body chemoreflex dysfunction during heart failure. Experimental Physiology, 2015, vol. 100, no. 2, pp. 124–129. https://doi.org/10.1113/expphysiol.2014.079517
15. Backer A. J. Adrenergic signaling in heart failure: a balans toxic and protective effects. Pflügers Archiv – European Journal of Physiology, 2014, vol. 466, no. 6, pp. 1139–1150. https://doi.org/10.1007/s00424-014-1491-5
Review
Views: 835
Email this article 