Use of hemostatic drug Spongostan as a carrier of mesenchymal stem cells in the treatment of experimental urinary incontinence in rats
https://doi.org/10.29235/1561-8323-2019-63-4-457-465
Abstract
The adhesion and proliferation of rat adipose-derived mesenchymal stem cells (MSCs) as part of biocomposite with haemostatic drug Spongostan Absorbable Haemostatic Gelatin Powder as well as its regenerative efficiency after its introduction into the periurethral region in rats with experimental urinary incontinence were studied. It was found that MSCs rapidly adhere to the surface of the carrier and actively proliferate. The periurethral injection of biocomposite (Spongostan + MSCs) quickly and effectively recovers the urine continence function in animals that have undergone modeling of incontinence by urethrolysis. Spongostan hydrated in the ratio of 1 :24 inphosphate-saline buffer has a volume-forming effect in the urethra of experimental animals.
Supporting agencies: The work is done within the frameworkof the subprogram «Innovative Biotechnology–2020» of the State Program «Science-based Technologies and Technics» for 2016–2020 (project 32)
About the Authors
Sergei V. Pinchuk
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Pinchuk Sergei Vladimirovich – Ph. D. (Biology), Senior researcher
27, Akademicheskaya Str., 220072, Minsk
Alla Yu. Molchanova
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Molchanova Alla Yur’evna – Ph. D. (Biology), Head of the Laboratory
28, Akademicheskaya Str., 220072, Minsk
Irina B. Vasilevich
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Vasilevich Irina Borisovna – Researcher
27, Akademicheskaya Str., 220072, Minsk
Irina P. Zhavoronok
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Zhavoronok Irina Petrovna – Ph. D. (Biology), Senior researcher
28, Akademicheskaya Str., 220072, Minsk
Elena I. Pekhtereva
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Pehtereva Elena Ivanovna – Juniour researcher
28, Akademicheskaya Str., 220072, Minsk
Olga A. Antipova
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Antipova Olga Aleksandrovna – Juniour researcher
28, Akademicheskaya Str., 220072, Minsk
Iosif F. Zalutski
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Corresponding Member
Belarus
Corresponding Member
Igor D. Volotovski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Volotovski Igor Dmitrievich – Academician, D. Sc. (Biology), Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
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