Role of potential-dependent potassium channels in forming the cellular response to the electrical stimulation of cells in the culture

Usage of electric fields for forming a certain cellular response finds application in various fields of biology and medicine. The efficiency of the methods based on the electric field action was discussed repeatedly. However, the process of developing stimulation protocols is being complicated by the absence of the specification of the precise mechanism of the electric stimulation action upon cells in the culture. Thus, the identification of signal transduction mechanism in cells under the electric field action is of current interest. The objective of this work is to investigate the role of potassium potential-dependent channels in forming the cellular response at long-term electrical stimulation. Studies were carried out using glioma cells C6. The pulsed electric field with the strength of 3-20 V/m, with 2 ms biphase pulses and with a frequency of 10 Hz was used for electric stimulation. 4-Aminopyridine was used for inhibitory analysis. It was shown that the change in the membrane potential under the electric field action takes place involving the potassium potential-dependent ion channels. It was revealed that the use of a potassium potential-dependent ion channels inhibitor partially levels the field effects. Thus, potassium potential-dependent channels play a significant role in the processes of signal transduction in cells at long-term electrical stimulation of the culture of cells.

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