SCREENING OF THROMBOXANE SYNTHASE PEPTIDOMIMETICS BY PEPTIDE PHAGE DISPLAY
Abstract
In order to identify potential inhibitors and modulators of thromboxane synthase, its molecular cloning, heterologous expression, isolation, and purification have been carried out. The recombinant protein is isolated in the homogenous state and possesses the functional activity. Peptide with an amino acids sequence SGVYKVLYDWQHGGF is identified using the random dodecapeptides phage library. It is revealed that this peptide has a great similarity to thrombopoietin peptidomimetic. This allows one to suppose the role of this hormone in the thromboxane synthase regulation.
About the Authors
D. O. DORMESHKIN
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
A. V. SVIRID
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
A. A. GILEP
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
S. A. USANOV
Институт биоорганической химии НАН Беларуси, Минск
Belarus
Belarus
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