Interaction between reactive oxygen species and gallocyanine under neutrophil activation
https://doi.org/10.29235/1561-8323-2019-63-6-730-735
Abstract
Reactive oxygen species (ROS) have a crucial role in human physiological and pathophysiological processes. Prolonged exposure to high ROS concentrations may lead to cardiovascular, neurodegenerative, etc. diseases. In this study, gallocyanine has been proposed to register the ROS production. The gallocyanine spectral properties changes under ROS (•О2ˉ, H2O2) and reactive halogen (HOCl) species are analyzed. It is shown that the dye is oxidized in solution, both under the action of ROS and reactive halogen species. Based on the data obtained, it is possible to suggest that superoxide anion radicals make a major contribution to chemical conversion of the dye in suspensions of activated neutrophils. It is that gallocyanine can be used to assess the functional activity of neutrophils, namely, the NADPH-oxidase, as well as to design and test novel therapeutic agents for diseases associated with developing oxidative stress.
About the Authors
V. E. Lutsenko
Belarusian State University
Russian Federation
Russian Federation
Lutsenko Veronika Evgenievna – Postgraduate student.
4, Nezavisimosti Ave., 220030, Minsk
D. V. Grigorieva
Belarusian State University
Russian Federation
Russian Federation
Grigorieva Daria Vladimirovna – Ph. D. (Biology), Senior researcher.
4, Nezavisimosti Ave., 220030, Minsk
I. V. Gorudko
Belarusian State University
Belarus
Belarus
Gorudko Irina Vladimirovna – Ph. D. (Biology), Associate professor.
4, Nezavisimosti Ave., 220030, Minsk
A. V. Sokolov
Institute of Experimental Medicine
Russian Federation
Russian Federation
Sokolov Alexey Viktorovich – D. Sc. (Biology), Professor, Head of the Laboratory.
12, Academician Pavlov Str., 197376, Saint Petersburg
O. M. Panasenko
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Russian Federation
Russian Federation
Panasenko Oleg Mikhailovich – D. Sc. (Biology), Professor, Head of Department.
1а, Malaya Pirogovskaya Str., 119435, Moscow
S. N. Cherenkevich
Belarusian State University
Russian Federation
Russian Federation
Cherenkevich Sergey Nikolaevich – Academician, D. Sc. (Biology), Professor.
4, Nezavisimosti Ave., 220030, Minsk
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