Activation of the peroxidase gene in potato plants expressing the gene of antimicrobial peptide MsrA1
https://doi.org/10.29235/1561-8323-2020-64-3-325-331
Abstract
Antimicrobial peptide MsrA1 is a synthetic hybrid molecule based on cecropin A from giant silk moth larvae and on melittin from melliferous bee venom. Transgenic potato plants of the Belarusian variety Odyssey with the constitutive expressiоn of msrA1 gene are shown to exhibit increased resistance to fungal pathogens Phytophthora infestans and Alternaria solani. Peroxidase genes expression studies using cDNA-PCR and DNA sequencing revealed the activation of the POX peroxidase gene in transgenic plants in the absence of pathogens. This may be indirect evidence of the increased formation of reactive oxygen species, which may explain special resistance to fungal pathogens. The data obtained also confirm a possible role of intracellular antimicrobial peptide in making the plants more resistant to oxidative stress by the way of activation of the host plant defense system.
About the Authors
A. Yu. Misyukevich
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Misiukevich Ala Yu. – Junior researcher
27, Akademicheskaya Str., 220072, Minsk
T. A. Gapeeva
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Gapeeva Тамаra A. – Ph. D. (Biology), Senior researcher
27, Akademicheskaya Str., 220072, Minsk
T. G. Tretyakova
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Tretyakova Tatiana G. – Junior researcher
27, Akademicheskaya Str., 220072, Minsk
T. V. Semanyuk
Scientific and Practical Center of the National Academy of Sciences of Belarus on Potato and Fruit and Vegetable Growing
Belarus
Belarus
Semanyuk Tamara V. – Senior Researcher
2а, kovalev Str., 223013, Samokhvalovichi, Minsk district, Minsk region
I. D. Volotovski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Volotovski Igor D. – Academician, D. Sc. (Biology), Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
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