Preview

Doklady of the National Academy of Sciences of Belarus

Advanced search

Morphological-cultural and molecular genetic features of collection strains of mycoris mushrooms Phialocephala fortinii and Pezicula sp.

https://doi.org/10.29235/1561-8323-2020-64-5-567-573

Abstract

A metagenomic analysis of the endophytic microflora of Vaccinium corymbosum L. and Vaccinium myrtillus L. root systems was carried out. Two dominant species of micromycetes forming ericoid mycorrhiza were identified - Phialocephala fortinii C. J. K. Wang & H. E. Wilcox and Pezicula sp. Tul. & C. Tul. Pure cultures of mycorrhizal fungi were prepared, a comprehensive morphological and genetic assay of the strains was carried out. Based on the results of genetic-taxonomic analysis, the assumption of the polyphyletic origin of species belonging to Phialocephala and Pezicula is confirmed.

About the Authors

Ya. S. Kamelchuk
Polessky State University
Belarus

Kamelchuk Yanina S. - Master of Sciences (Biology), Postgraduate student, Polessky State University.
23, Dneprovskoy flotilii Str., 225710, Pinsk.



O. Yu. Baranov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus

Baranov Oleg Yu. - D. Sc. (Biology), Assistant professor, Head of the Department, Forest Research Institute of the National Academy of Sciences of Belarus.
71, Proletarskaya Str., 246050, Gomel.



P. S. Kiryanov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus

Kiryanov Pavel S. - Junior researcher, Forest Research Institute of the National Academy of Sciences of Belarus.
71, Proletarskaya Str., 246050, Gomel.



V. E. Padutov
Forest Research Institute of the National Academy of Sciences of Belarus
Belarus

Padutov Vladimir E. - Corresponding Member, D. Sc. (Biology), Head of the Laboratory, Forest Research Institute of the National Academy of Sciences of Belarus.
71, Proletarskaya Str., 246050, Gomel.



References

1. Kamel'chuk Ya. S., Laman N. A. Features of cultivation in vitro endomicorized mushrooms from the roots of the heather family (Ericaceaejuss.) Botanika (issledovaniya) [Botany (research)]. Minsk, 2018, vol. 47, pp. 110-115 (in Russian).

2. Litvinov M. A. Methods for studying soil microscopic fungi. Leningrad, 2009. 121 p. (in Russian).

3. Zvyagintsev D. G. Methods of soil microbiology and biochemistry. Moscow, 1991. 302 p. (in Russian).

4. Boyer E. P., Ballington G. R., Hamland C. M. Endomycorrhizae of Vaccinium corymbosum L. in North Carolina. Journal of American Society Horticultiral Science, 2012, vol. 107, no. 5, pp. 751-754.

5. Peterson R. L., Wagg C., Pautler M. Associations between microfungal endophytes and roots: do structural features indicate function? Botany, 2008, vol. 86, no. 5, pp. 445-456. https://doi.org/10.1139/b08-016

6. Grunig Ch. R., Queloz V., Sieber Th. N., Holdenrieder O. Dark septate endophytes (DSE) of the Phialocephala for-tinii s. l. - Acephala applanata species complex in tree roots: classification, population biology, and ecology. Botany, 2008, vol. 86, no. 12, pp. 1355-1369. https://doi.org/10.1139/b08-108

7. Currah R. S., Tsuneda A. Vegetative and reproductive morphology of Phialocephala fortinii (Hyphomycetes, Mycelium radicisatrovirens) in culture. Transactions of the Mycological Society of Japan, 1993, vol. 34, no. 3, pp. 345-356.

8. Currah R. S., Tsuneda A. Morphology and ecology of Phialocephala fortinii in roots of Rhododendron brachycar-pum. Canadian Journal of Botany, 1993, vol. 71, no. 12, pp. 1639-1644. https://doi.org/10.1139/b93-199

9. Wang X., Liu C., Huang L., Bengtsson-Palme J., Chen H., Zhang J., Cai D., Li J. ITS1: a DNA barcode better than ITS2 in eukaryotes? Molecular Ecology Resources, 2014, vol. 15, no. 3, pp. 573-586. https://doi.org/10.1111/1755-0998.12325

10. Basic local alignment search tool (BLAST NCBI). Available at: https://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed 21 January 2020).

11. Chen C., Verkley G. J. M., Sun G., Groenewald J. Z., Crous P. W. Redefining common endophytes and plant pathogens in Neofabraea, Pezicula, and related genera. Fungal Biology, 2015, vol. 120, no. 11, pp. 1291-1322. https://doi.org/10.1016/j.funbio.2015.09.013


Review

Views: 12348


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1561-8323 (Print)
ISSN 2524-2431 (Online)