Genogeography of norway spruce (Picea abies (L.) Karst.) according to the analysis of cytoplasmic DNA
https://doi.org/10.29235/1561-8323-2021-65-4-439-447
Abstract
Norway spruce (Picea abies (L.) Karst.) is one of the main forest-forming species in Belarus. It plays important economic, ecological and social roles. The spruce forest of the region is characterized by a complex history of the formation of its population genetic structure. The aim of this study was the genogeographic analysis of P. abies populations and the description of regional features of its gene pool in Belarus. Molecular genetic analysis of microsatellite (SSR) loci of chloroplast DNA and mt15-D02 locus of mitochondrial DNA of Norway spruce was carried out for samples from 57 naturally originated forest stands. We identified 19 allelic variants of the Pt63718, Pt26081, Pt71936 cpDNA loci and two allelic variants of mt15-D02 mtDNA. The geographical distribution of the alleles has been described and the regional features of the genogeographic differentiation of the spruce forests have been considered. The southern border of the continuous distribution area of P. abies and its island localities lie in the south of Belarus (Brest and Gomel regions). The frequency of occurrence of individual allelic variants of cpDNA SSR loci has the most pronounced deviations from the average values for the whole country in those regions. Analysis of cpDNA showed the presence of certain regional features of the genogeographic structure of the spruce forest in the “south-north” and “west-east” directions. According to mitochondrial DNA analysis higher concentration of P. abies trees of southern (Carpathian) origin is observed in the southwest of Belarus. On the contrary northern (Boreal) origin dominates in the rest of the country. The results of performed genogeographic analysis of Norway spruce populations can serve as a basis for improving the forest seed zoning of the tree species.
About the Authors
V. E. Padutov
Institute of Forest of the National Academy of Sciences of Belarus
Belarus
Belarus
Padutov Vladimir E. – Corresponding Member, D. Sc. (Biology), Associated professor, Head of the Department
71, Proletarskaya Str., 246050, Gomel
D. I. Kagan
Institute of Forest of the National Academy of Sciences of Belarus
Belarus
Belarus
Каgаn Dmitry I. – Ph. D. (Biology), Head of the Laboratory
71, Proletarskaya Str., 246050, Gomel
S. I. Ivanovskaya
Institute of Forest of the National Academy of Sciences of Belarus
Belarus
Belarus
Ivanovskaya Svetlana I. – Ph. D. (Biology), Senior research
71, Proletarskaya Str., 246050, Gomel
O. Yu. Baranov
Institute of Forest of the National Academy of Sciences of Belarus
Belarus
Belarus
Baranov Oleg Yu. – D. Sc. (Biology), Associated professor, Head of the Laboratory
71, Proletarskaya Str., 246050, Gomel
T. S. Markevich
Institute of Forest of the National Academy of Sciences of Belarus
Belarus
Belarus
Markevich Tatyana S. – Ph. D. (Biology), Researcher
71, Proletarskaya Str., 246050, Gomel
References
1. Sarnatsky V. V. Particularity of farming in conditions of extreme damage of forest ecosystems in Belarus. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya biyalagichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Biological series, 2015, no. 1, pp. 21–25 (in Russian).
2. Parfenov V. I. Conditionality of distribution and adaptation of plant species at the boundaries of areas. Minsk, 1980. 208 p. (in Russian).
3. Elovicheva Ya. K. Palynology of Belarus: in 4 parts. Minsk, 2018. 831 p. (in Russian).
4. Kovalevich O. A., Kagan D. I., Padutov V. E. Genogeographic features of the distribution of pedunculate oak populations in Belarus. Sovremennoe sostoyanie, tendentsii razvitiya, ratsional’noe ispol’zovanie i sokhranenie biologicheskogo raznoobraziya rastitel’nogo mira: materialy Mezhdunarodnoi nauchnoi konferentsii [Current state, development trends, rational use and conservation of biological diversity of the plant world: materials of the International Scientific Conference]. Minsk, 2014, pp. 328–332 (in Russian).
5. Kavender-Behres D., Manos P., Khipp E. H. The Blossom of Oaks. V mire nauki = In the world of science, 2020, no. 10, pp. 4–13 (in Russian).
6. Maghuly F., Nittinger F., Pinsker W., Praznik W., Fluch S. Differentiation among Austrian populations of Norway spruce [Picea abies (L.) Karst.] assayed by mitochondrial DNA markers. Tree Genetics and Genomes, 2007, vol. 3, no. 3, pp. 199–206. https://doi.org/10.1007/s11295-006-0055-z
7. Ran J.-H., Wei X.-X., Wang X.-Q. Molecular Phylogeny and Biogeography of Picea (Pinaceae): Implications for Phylogeographical Studies Using Cytoplasmic Haplotypes. Molecular Phylogenetics and Evolution, 2006, vol. 41, no. 2, pp. 405–419. https://doi.org/10.1016/j.ympev.2006.05.039
8. Litkowiec M., Dering M., Lewandowski A. Utility of two mitochondrial markers for identification of Picea abies refugial origin. Dendrology, 2009, vol. 61, pp. 65–71.
9. Dering M., Misiorny A., Lewandowski A., Korczyk A. Genetic and historical studies on the origin of Norway spruce in Białowieza Primeval Forest in Poland. European Journal of Forest Research, 2012, vol. 131, no. 2, pp. 381–387. https://doi.org/10.1007/s10342-011-0510-8
10. Potokina E. K., Orlova L. V., Vishnevskaya M. S., Alekseeva E. A., Potokin A. F., Egorov A. A. Genetic differentiation of spruce populations in northwest Russia according to the results of microsatellite loci analysis. Russian Journal of Genetics: Applied Research, 2013, vol. 3, no. 5, pp. 352–360. https://doi.org/10.1134/s2079059713050080
11. Aizawa M., Yoshimaru H., Ogawa H., Goto S., Kaji M. Natural hybridization of Yezo and Sakhalin spruce in central Hokkaido, revealed by DNA markers with contrasting modes of inheritance. Plant Species Biology, 2016, vol. 31, no. 3, pp. 188–195. https://doi.org/10.1111/1442-1984.12101
12. Mudrik E. A., Polyakova T. A., Shatokhina A. V., Bondarenko G. N., Politov D. V. Spatial distribution of intron 2 of nad1 gene haplotypes in populations of norway and siberian spruce (Picea abies–P. obovata) species complex. Russian Journal of Genetics, 2015, vol. 51, no. 10, pp. 957–965. https://doi.org/10.1134/s1022795415100129
13. Padutov V. E., Baranov O. Yu., Voropaev E. V. Methods of molecular and genetic analysis. Minsk, 2007. 176 p. (in Russian).
14. Yurkevich I. D., Golod D. S., Parfenov V. I. Types and associations of spruce forests (according to research in the BSSR). Minsk, 1971. 350 p. (in Russian).
15. Yurkevich I. D., Golod D. S., Parfenov V. I. Typology and shape composition of spruce forests of Belarus. Voprosy lesovedeniya [Problems of forestry]. Krasnoyarsk, 1970, pp. 130–185 (in Russian).
16. Tsuda Y., Chen J., Stocks M., Källman T., Sønstebø J. H., Parducci L., Semerikov V., Sperisen C., Politov D., Ronkainen T., Valiranta M., Vendramin G. G., Tollefsrud M. M., Lascoux M. The extent and meaning of hybridization and introgression between Siberian spruce (Picea obovata) and Norway spruce (Picea abies): cryptic refugia as stepping stones to the west? Molecular Ecology, 2016, vol. 25, no. 12, pp. 2773–2789. https://doi.org/10.1111/mec.13654
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