Dependence of expression of genes controlling calcium signaling on gravistimulation in cells of tomato apical leaves and treatment by etephon
https://doi.org/10.29235/1561-8323-2021-65-4-456-465
Abstract
The sensitivity of expression at the level of transcription of genes encoding proteins involved in calcium signal transduction to gravistimulation was revealed using real-time RT-PCR. For three genes SCA2, РВР2, САМ2, the increase in the transcript formation was shown at early response stages, starting from 15–60 minute gravistimulus. The treatment of plants before the start of gravistimulation with an ethephon (source of exogenous ethylene) led to a change in the modulation of expression of the studied genes in response to gravistimulus. The role of calcium metabolism in realization of final steps of gravitropism reaction is considered.
Keywords
tomato (Lycopersicum esculentum L.),
gravitropism,
gene expression,
ethylene,
ethephon,
calcium ions,
сalcium-transporting ATPase,
calmodulin,
PID-binding protein
Supporting agencies: The work was supported by the Belarusian Republican Foundation for Fundamental Research (project Б19M-109).
About the Authors
S. V. Sukhaveyeva
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Sukhaveyeva Sviatlana V. – Researcher
27, Akademicheskaya Str., 220072, Minsk
A. М. Kаbachevskaya
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Kabachevskaya Alena M. – Ph. D. (Biology), Leading researcher
27, Akademicheskaya Str., 220072, Minsk
I. D. Volotovski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Volotovski Igor D. – Academician, D. Sc. (Biology), Professor, Chief researcher
27, Akademicheskaya Str., 220072, Minsk
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