Modifying the tumor effects in the lungs of laboratory mice with lichen extracts
https://doi.org/10.29235/1561-8323-2021-65-4-474-483
Abstract
The anti-tumor properties of common lichens of Belarus: Hypogymnia physodes, Ramalina pollinaria, Cladonia arbuscula, Evernia prunastri were studied using the models of spontaneous and induced carcinogenesis in mice Af lungs. Without exposing to a carcinogen in 20 weeks after taking aqueous lichen extracts (1 g/l) of 4 species for 28 days, only C. arbuscula showed the pro-carcinogenic properties almost doubling the average number of pulmonary adenomas per mouse. R. pollinaria and E. prunastri did not provoke spontaneous carcinogenesis and had a corrective effect significantly reducing the number of adenomas per mouse by 21 and 45 %, respectively. To carry out induced carcinogenesis, after 2 weeks of taking aqueous extracts, mice were injected intraperitoneally with urethane (1 g/kg). The analysis of the total number of adenomas per mouse showed a pro-tumor tendency for C. arbuscula, and the analysis of the number of adenomas of 1 mm and more showed that for H. physodes. The extracts do not affect the proportion of urethane adenomas of various sizes in their total number, but increase the incidence of high-yield adenomas of various sizes; at the same time, H. physodes has a much greater pro-carcinogenic activity according to the incidence of high-yield adenomas of 1 mm or more. The analysis of the total number of urethane adenomas per mouse, as well as adenomas of 1 mm showed an anti-tumor tendency for R. pollinaria and a pro-tumor tendency for E. prunastri. Taking E. prunastri increased the incidence of high-yield adenomas of various sizes, along with a notable increase in the incidence of low-yield adenomas. In the case of R. pollinaria, the increase in the incidence of low-yield adenomas was supplemented by a sharp decrease in the incidence of high-yield adenomas. As a result of the experiment, E. prunastri has exhibited both the pro-carcinogenic activity and the anti-tumor properties, but R. pollinaria has revealed a pronounced anti-tumor potential.
About the Authors
S. V. Goncharov
Institute of Radiobiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Goncharov Sergey V. – Researcher
4, Fedyuninsky Str., 246007, Gomel
I. A. Cheshik
Institute of Radiobiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Cheshik Igor A. – Ph. D. (Medicine), Associate professor, Director
4, Fedyuninsky Str., 246007, Gomel
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