REGULATION OF THE PROLIFERATIVE ACTIVITY AND CHEMORESISTANCE IN TUMOR CELLS BY SODIUM ASCORBATE
https://doi.org/10.29235/1561-8323-2018-62-1-93-100
Abstract
The effect of ascorbate in physiological concentrations on the proliferative activity and chemoresistance in human larynx carcinoma HEp-2 cells was studied. Ascorbate in a concentration of 60 μM was found to increase the cancer cells proliferation rate 1.5 times. Ascorbate changes the functional state of the cancer cells, thereby increasing their resistance to doxorubicin and thymoquinone. It was shown that apocynin (NADPH oxidase inhibitor) blocks the stimulating effect of the antioxidant. The results obtained suggest that reactive oxygen species produced by NADPH oxidase participate in the mechanism of cell adaptive response induced by ascorbate.
Supporting agencies: The work is sponsored by the Belarusian Republican Foundation for Fundamental Research (grant No. М16Р-022) and by the Russian Foundation for Basic Research (grant no. 16-54-00050).
About the Authors
Grigory G. Martinovich
Belarusian State University
Belarus
Belarus
D. Sc. (Biology), Assistant Professor, Head of the Department
4, Nezavisimosti Ave., 220030, Minsk
Irina V. Martinovich
Belarusian State University
Belarus
Belarus
Senior researcher
4, Nezavisimosti Ave., 220030, Minsk
Aleksandra V. Vcherashniaya
Belarusian State University
Belarus
Belarus
Junior researcher
4, Nezavisimosti Ave., 220030, Minsk
Nikolai K. Zenkov
Research Institute of Experimental and Clinical Medicine
Russian Federation
Russian Federation
D. Sc. (Biology), Leading researcher
2, Academician Timakov Str., 630117, Novosibirsk
Elena B. Menshchikova
Research Institute of Experimental and Clinical Medicine
Russian Federation
Russian Federation
D. Sc. (Medicine), Assistant Professor, Chief researcher
2, Academician Timakov Str., 630117, Novosibirsk
Sergei N. Cherenkevich
Belarusian State University
Belarus
Belarus
Academician, D. Sc. (Biology), Professor
4, Nezavisimosti Ave., 220030, Minsk
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